Researchers should have respect for the originator of the cell lines.

The esophageal squamous cell carcinoma cell lines (KYSE series) have been established and characterized in our laboratory (1–3). These cell lines have proved to be useful and appropriate research materials in the field of esophageal squamous cell carcinoma research. Thus, these cell lines have been provided to many collaborative researchers by us without strict regulation of their use. These cell lines have also been donated to certain cell banks. However, I believe that there is ethical rule that researchers should not use the cell lines and provide them to other researchers without the permission of the originator of the cell lines. There was a report that some cell lines were already crosscontaminated because of the use of a cell line without strict quality control in the laboratory (4). There may exist a relaxed way of thinking in the usage of the cell lines in these kinds of laboratories. For these reasons, I read Tong et al.’s article, regarding Aurora-A expression in esophageal squamous cell carcinoma (5), with great interest. I found that there were several mistakes or inappropriate issues in the paper. They stated that they used KYSE-2, 9706, KYSE-450, KYSE-180, Colo-680, KYSE-150, KYSE-140, KYSE-410, KYSE-30, KYSE-510, T12, and KYSE-70 in Materials and Methods without any comment on the source of cell lines. Furthermore, they changed the name Colo-680 and 9706 to KYSE-680 and KYSE-9706, respectively, in the text and figures. Indeed, I established KYSE-30, 70, 140, 150, 180, 410, 450, and 510 cells; however, I have never established KYSE-2, KYSE-680, and KYSE-9706. Thus, the following questions were raised. Were KYSE-2, KYSE-9706 (or 9706), and KYSE-680 cells established by the authors? If so, the authors should indicate the references for these cells. I think the name of KYSE-2 and KYSE-9706 is unusual. The readers of the journal may misinterpret that these cell lines are included in the KYSE series. Were these names mislabeled typographic errors? If so, what were the exact names of these cells? Which is correct, the name Colo-680 or KYSE-680? If the name of Colo-680 is correct, was this cell established from esophageal squamous cell carcinoma? The name ‘‘Colo’’ usually represents colon cancer. I have no record of depositing these cells with the authors. Were KYSE-30, 70, 140, 150, 180, 410, 450, and 510 cell lines obtained from a certain cell bank? If so, they should indicate the exact name of the source bank. This article has some discrepancies and I am afraid that there may exist cross-contamination of the cells.